Abstract
ABSTRACT Reversible and irreversible aspects of polyclonal IgG adsorption onto glass beads were studied using 125I radiolabeled proteins for bulk concentrations as low as 4× 10−4 mg ml−1. A true thermodynamic equilibrium was reached in the low bulk concentration domain, and a saturation of 0.1μg cm−2 was noticed for concentrations ranging from 0.03 to 0.37mg ml−1. The decrease of the amount of desorbed IgG's with increasing bulk concentrations in the plateau domain taken together with the impossibility to adjust desorption kinetics using a single exponential law, allowed us to propose an adsorption mechanism in which two protein populations i.e. rapidly (state B) and slowly (state C) desorbed molecules are involved
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
