Adrenergic modulation of the blood neutrophilia induced by platelet activating factor in rats

Abstract

The involvement of catecholamines in blood neutrophilia observed after administration of platelet activating factor (PAF) to rats was studied. Intravenous (i.v.) injection of PAF (4 μg/kg) into naive rats more than doubled the number of neutrophils after 1 h. In contrast, PAF failed to induce neutrophilia in both adrenalectomized rats and those which had their catecholamine stores depleted by reserpine. PAF-induced neutrophilia was not inhibited by the selective antagonism of α 1 and α 2-adrenoceptors with prazosin and yohimbine, respectively. However, pretreatment with the β-adrenoceptor antagonist, propranolol, significantly inhibited the phenomenon. Increase in the blood neutrophil counts was also achieved following the i.v. injection of the β-agonist, salbutamol (1 mg/kg, i.v.). This response was clearly sensitive to propanolol but was not modified in rats submitted to adrenalectomy or reserpine pretreatment. The results suggest that the blood neutrophilia induced by the i.v. administration of PAF in rats is dependent on the stimulation of β-adrenoceptors by catecholamines released from adrenal glands.