Adrenaline and cyclic AMP stimulated uptake of chloride and fluid secretion by isolated salivary glands of the lone star tick

Abstract

1. Copper (10 −3 M) effectively inhibits 10 −5 M adrenaline stimulated uptake of 36Cl by isolated salivary glands of the lone star tick. 2. 2,4-dinitrophenol (DNP) (10 −3 M), ouabain (10 −3 M), and harmaline (10 −3 M) partially depress adrenaline stimulated uptake, but an antagonist of nerve postsynaptic β-receptors, propranolol (10 −3 M), is without effect. 3. Copper, DNP, and ouabain partially inhibit 10 −2 M cyclic AMP stimulated uptake of 36Cl by isolated tick salivary glands, but 10 −3 M harmaline enhances uptake. 4. A combination of high concentrations of cyclic AMP and theophylline stimulate fluid secretion by isolated tick salivary glands. 5. The significance of these findings to the control of ion movements into and across tick salivary glands and their significance mechanisms of fluid secretion is discussed.