ADP-ribosyltransferase activity during the friend virus-induced murine erythroleukemia cell differentiation.

V L Pulito,D L Miller,S Sassa,T Yamane

doi:10.1016/s0021-9258(17)43722-7

Abstract

A variety of chemical agents that are known to induce erythrodifferentiation in the Friend virus-induced murine erythroleukemia (MEL) cell have been suggested to mediate DNA cleavage in cultured cells prior to differentiation. The activation of the nuclear enzyme, ADP-ribosyltransferase, depends upon the presence of single strand breaks in DNA. If dimethyl sulfoxide (Me2SO) causes DNA breakage, it would be expected that the activity of ADP-ribosyltransferase would increase. A study of ADP-ribosyltransferase activity during cell growth indicates that both Me2SO-treated and untreated MEL cells exhibit a similar increase in the enzyme activity but the increase in Me2SO-treated cells is delayed by a few hours. When examined at comparable stages of growth, both treated and untreated cells show almost identical levels of enzyme activity. The present data thus do not support the contention that Me2SO induces DNA breakage in the MEL cells.

Highlights

10314, and [I The Rockefeller University, New York, New York 10021 the formationof poly(ADP-ribose)-modifiednuclear proteins from NAD’, might be increased, because in several systems it has been demonstrated that DNA breakage increases the
A study of ADP-ribosyltransferase activity during cell growth indicates that both Me&O
murine erythroleukemia (MEL) cells are believed to be erythroid precursors arrested at the proerythroblast level of development but irreversibly committedtoerythrodifferentiation[3].It is possible that thisstage of development is already an advancedstage of erythrodifferentiation along the “suicidal path”, whereas the DNA breakage and chromosome rearrangements necessary for differentiation have alreadytaken place at anearlier phase of development

Summary

Introduction

10314, and [I The Rockefeller University, New York, New York 10021 the formationof poly(ADP-ribose)-modifiednuclear proteins from NAD’, might be increased, because in several systems it has been demonstrated that DNA breakage increases the. The activation of the nuclear enzyme,ADP-ribosyltransferase, depends upon the presence of single strand breaks in DNA. If dimethyl sulfoxide (Me2SO)causes DNA breakage, it would be expected that the activity of ADP-ribosyltransferase

Results

Conclusion