Abstract
Objective:To observe the anti-tumor effect of adoptive immunotherapy with CTL induced by PSA-pulsed dendritic cell vaccine against prostate cancer in mice.Methods:Nude mice were s.c.inoculated with LNCaP tumor cells to establish prostate cancer model.CTL induced in vitro by different peptide-pulsed dendritic cells(Non-DC,Ova-DC,Lys-DC and PSA-DC) were used for adoptive immunotherapy of experiment nude model through vena caudalis on d 15 and d 22 after inoculation.The survival of nude mice and the sizes of tumors were observed with d 50 after the initial treatment as the endpoint.Results:The tumor sizes in Non-DC,Ova-DC,Lys-DC and PSA-DC groups on d 30 and d 50 after adoptive immunotherapy were significantly larger than that at d 15 after inoculation(P0.01).The tumors sizes in Lys-DC and PSA-DC groups were significantly smaller than those in the Non-DC and Ova-DC groups(P0.01).The survival periods of animals in Lys-DC and PSA-DC groups were significantly longer than those in the Non-DC and Ova-DC groups(P0.01).Conclusion:Adoptive immunotherapy with CTL induced by DC-based PSA vaccine can inhibit the growth of prostate cancer LNCaP cells in nude mice and increase the survival of the animals.
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