Administration of Solo Garlic Extract (Allium sativum Var. Solo Garlic) Reduced Low Density Lipoprotein and Increased Superoxide Dismutase than Control in Male Wistar Rats (Rattus norvegicus) Fed with High Fat Diet

Abstract

Introduction: Obesity increases the release of ROS and cause oxidative stress. Solo Garlic Extract (Allium sativum Var. Solo Garlic) has secondary metabolites such as flavonoids, ajoene, organosulfur, allicin, phenol tannins and other compounds which are known to have antioxidant, anti-inflammatory activity and can improve blood lipid profiles. This study aims to prove the effect of solo garlic extract on LDL and SOD level in male Wistar rats fed with high-fat diet. Methods: This was true experimental research using post-test only control group design. The subjects were 30 male Wistar rats aged 2-3 months, weighing 150-200 grams that were randomly divided into 3 groups. The normal group (N) was given standard diet, the control group (C) was given high-fat diet and 1 cc of placebo, and the treatment group (T) was given high-fat diet and 0.2 gram in 1 cc of solo garlic extract, given once each day for 28 days. The blood was taken to examine LDL and SOD level after 28 days. Data was analyzed using One Way Anova. Results: The results showed that there was a significant difference in LDL: The N group (20.45 ug/mL), the C group (37.32 ug/mL) and The T group (24.44 ug/mL). The LDL in the T group was lower than that of the C group (p<0.001) and the LDL in the T group was higher than the N group (p<0.001). There was a significant difference in SOD between the N group (0.88 ng/mL), the C group (0, 89 ng/mL) and the T group (1.99 ng/mL). The SOD of the T group was higher than that of the C group (p<0.001) and the N group (p<0.001). Conclusion: Administration of 0.20 gram in 1cc/day of solo garlic extract reduced LDL level and increased SOD enzyme in male Wistar rats (Rattus norvegicus) fed with high-fat diet.