Abstract
The aim of the present study was to examine the effect of ovarian stimulation with increasing amounts of pregnant mare's serum gonadotrophin (PMSG) on preimplantation development of diploid parthenogenetic embryos in vitro. Administration of 5, 10 and 20 IU PMSG significantly increased the number of oocytes obtained per mouse in a dose-dependent manner. The amount of PMSG administered did not alter the proportion of degenerate oocytes. However, there was a significant decrease in the proportion of 8-cell/compacted embryos after 53 h of culture with the administration of increasing amounts of PMSG. Proportion of embryos reaching at the blastocyst stage after 79 h of culture was reduced significantly in both the 10 and 20 IU PMSG groups. Reduced blastocyst development after 96 h of culture, however, was significant only in the 20 IU PMSG group. Total blastocyst, trophectoderm and inner cell mass numbers were also reduced significantly with the administration of 20 IU PMSG. It is concluded on the basis of these observations that preimplantation development of diploid parthenogenetic oocytes, which depends virtually entirely on maternal molecules accumulated during oogenesis along with gene products derived from the maternal genome, is compromised with the administration of increasing amounts of PMSG.
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