Abstract
Objective To study the immune effects of IL-7, IL-21 as gene adjuvants, Poly(I: C) and CpG ODN for HIV vaccine. Methods Mouse interleukin 7 DNA adjuvant (pVR-IL7) and mouse interleukin 21 DNA adjuvant (pVR-IL21) were constructed. BALB/c mice received DNA prime-adenoviral vector boost immunization with pVR-HIVenv-Ad5-HIVenv alone or combined with pVR-IL7, pVR-IL21, Poly(I: C) and CpG ODN. Cellular and humoral immune responses were assessed by IFN-g enzyme-linked immunosorbent spot assay and enzyme-linked immunosorbent assay. Results Compared with those immunized with vaccines alone, the mice immunized with pVR-IL7 had increased specific cellular response (P<0.05), CpG ODN had synergic effects with IL7 as adjuvants(P<0.05) Conclusion IL7 but not IL21 can enhance the specific cellular immune response of Ad5-HIVenv in mice. Key words: HIV vaccine; Adjuvant; IL-7; IL-21
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