Abstract
The effect of M-Glucan, a β-1,3- and β-1,6-linked glucan from Saccharomyces cerevisiae , as an adjuvant in vaccines against furunculosis in Atlantic salmon ( Salmo salar L.) was studied. Vaccines consisted of formalin-killed cultures of Aeromonas salmonicida and in one case of a mixture of formalin-killed cultures of A. salmonicida and Vibrio salmonicida . M-Glucan was mixed with the vaccines to provide an administration dose of 0·5 mg/fish. Vaccines with or without glucan were injected into Atlantic salmon presmolts of 20-50 g, and the fish were challenged with A. salmonicida by a cohabitation method 3-46 weeks after vaccination. In all cases, vaccines supplied with M-glucan induced significantly higher protection against furunculosis than vaccines without M-Glucan ( P<0·015). When M-Glucan was injected alone, no protection was observed at 11 weeks after injection. Serum antibodies against A-layer protein, lipopolysaccharide, whole cells and extracellular products from A. salmonicida were measured by an ELISA-procedure. The non-adjuvanted vaccines induced antibodies to all antigens in Atlantic salmon. M-Glucan significantly enhanced the level of antibody against A-layer protein ( P=0·028), but not against lipopolysaccharide.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
