Abstract
Adjuvant-dependent immunity resulted in agglutinin activity against Aeromonas salmonicida in sera of convalescent fishes. Rainbow trout (Salmo gairdneri), brown trout (Salmo salar), and brook trout (Salvelinus fontinalis) that had recovered from natural furunculosis produced serum agglutinin antibodies predominately against a specific chromatographic fraction (EcF1) of the bacterium's extracellular product. EcF1 was resolved by ion-exchange chromatography from DEAE SephadexA-25. When EcF1 was emulsified in Freund's incomplete adjuvant and injected intraperitoneally, brook trout were protected against experimental challenge; brook trout vaccinated with EcF1 only were not protected against furunculosis. EcF1, examined by immunodiffusion tests, SDS – polyacrylamide gel electrophoresis, and Western blot immunoassays, shared certain properties with bacterial endotoxin. EcF1 and endotoxin preparations both contained immunoreactive lipopolysaccharides which indicated that adjuvant-dependent immunity to A. salmonicida is related to endotoxin.
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