Abstract
We aim to investigate the effects of adipose-derived stem cells (ASCs) transplantation on irradiation-induced skeletal muscle fibrosis. Sixty-four rabbits were randomly divided into ASCs group and PBS group followed by irradiation at unilateral hip with a single dose of 80 Gy. Nonirradiated side with normal skeletal muscle served as normal control. Skeletal muscle tissues were collected from eight rabbits in each group at 1 w, 4 w, 8 w, and 26 w after irradiation. Migration of ASCs was observed in the peripheral tissues along the needle passage in the injured muscle. The proportion of the area of collagen fibers to the total area in sections of ASCs group was lower than those of PBS groups at 4 w, 8 w, and 26 w after irradiation. Significant decrease was noted in the integrated optimal density of the transforming growth factor β1 (TGF-β1) in the ASCs group compared with those of PBS group at 4 w, 8 w, and 26 w after irradiation. Moreover, the expression of TGF-β1 was lower in the ASCs group compared to those of the PBS group at each time point determined by Western blot analysis. ASCs transplantation could alleviate irradiation fibrosis by suppressing the level of TGF-β1 in the irradiated skeletal muscle.
Highlights
IntroductionRadiotherapy has been commonly used to treat cancer with an aim of increasing the mean life expectancy of patients
Radiotherapy has been commonly used to treat cancer with an aim of increasing the mean life expectancy of patients.it usually induces normal tissue injury, among which radiation fibrosis is a common complication characterized by excess fibroblast proliferation and collagen fibers deposition [1,2,3,4]
adipose-derived stem cells (ASCs) were isolated from New Zealand rabbit and transplanted into the irradiated site to investigate the effect of ASCs implantation on the radiation-induced muscle fibrosis, based on which we provide a therapeutic strategy for ameliorating the irradiated skeletal muscle injury
Summary
Radiotherapy has been commonly used to treat cancer with an aim of increasing the mean life expectancy of patients It usually induces normal tissue injury, among which radiation fibrosis is a common complication characterized by excess fibroblast proliferation and collagen fibers deposition [1,2,3,4]. The total radiation field may include the neck, shoulder, axillary, and thoracic muscles. On this occasion, radiationinduced fibrosis is becoming a common and crippling side effect, leading to muscle imbalance with a lessened range of motion, as well as pain and dysfunction [9]. Patients undergoing prostate radiotherapy showed defecation urgency and fecal incontinence which was associated with the pelvic floor muscles and anorectal injury after radiation [10]
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