Adipose-derived mesenchymal stem cells formed acinar-like structure when stimulated with breast epithelial cells in three-dimensional culture.

Jing Tong,Lingyun Xiong,Quan Yuan,Jie Yang,Shan Mou,Raymund E Horch,Zhenxing Wang,Rongrong Wang,Jiaming Sun,Annika Weigand

doi:10.1371/journal.pone.0204077

Abstract

Lipotransfer has been applied in breast augmentation surgery for several years and the resident adipose-derived stem cells (ASCs) play an important role in enhancing fat graft survival. However, the interaction between ASCs and mammary epithelium is not fully understood. Many studies have shown that ASCs have a tumor-supportive effect in breast cancer. To the best of our knowledge, this is the first study on the effect of mammary epithelial cells on the human ASCs in 3D culture. ASCs were cultivated on matrigel in the conditioned medium (CM) prepared from a human breast epithelial cell line (HBL-100). The ASCs formed KRT18-positive acini-like structures after stimulation with breast epithelial cells. The expression of epithelial genes (CDH1 and KRT18) was up-regulated while the expression of mesenchymal specific genes (CDH2 and VIM) was down-regulated as determined by qRT-PCR. The stemness marker (CD29) and angiogenic factors (CD31 and VEGF) were also down-regulated as examined by immunofluorescence. In addition, the CM obtained from HBL-100 enhanced the migration and inhibited the adipogenic differentiation of ASCs. These results demonstrate that ASCs have the ability to transform into epithelial-like cells when cultured with mammary epithelial cells. Given these observations, we infer that ASCs have a positive effect on lipotransfer, not only due to their ability to secrete growth factors, but also due to their direct participation in the formation of new breast tissue.

Highlights

Breast augmentation with lipotransfer has recently become one of the most frequently demanded operations in aesthetic surgery
There were few CD34, CD14 and CD45-positive cells suggesting that there were few endothelial progenitors and hematopoietic cells (Fig 1E). These findings showed that the cells isolated met the minimal criteria to be defined as adipose-derived stem cells (ASCs) [14]
This study found that ASCs formed acinar-like structures and showed characteristics of epithelial differentiation when stimulated by the breast epithelial cell line HBL-100 in 3D

Summary

Introduction

Breast augmentation with lipotransfer has recently become one of the most frequently demanded operations in aesthetic surgery. Characteristics of the recipient area, the processing of the lipotransplant tissue conditions in the breast, and local angiogenesis have been found to affect the outcome of lipotransfer. From a technical point of view fat transfer is limited by the unpredictable and variable post operational absorption rate [1]. Adipose-derived mesenchymal stem cells formed acinar-like structure the Science Foundation of Wuhan Union Hospital (2016ZYCX034) to Zhenxing Wang and the National Natural Science Foundation of China (No 81501688) to Quan Yuan

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