Abstract
To explore the effects of adipose tissue-derived stem cells (ADSCs) on the proliferation and invasion of pancreatic cancer cells in vitro and the possible mechanism involved, ADSCs were cocultured with pancreatic cancer cells, and a cell counting kit (CCK-8) was used to detect the proliferation of pancreatic cancer cells. ELISA was used to determine the concentration of stromal cell-derived factor-1 (SDF-1) in the supernatants. RT-PCR was performed to detect the expression of the chemokine receptor CXCR4 in pancreatic cancer cells and ADSCs. An in vitro invasion assay was used to measure invasion of pancreatic cancer cells. SDF-1 was detected in the supernatants of ADSCs, but not in pancreatic cancer cells. Higher CXCR4 mRNA levels were detected in the pancreatic cancer cell lines compared with ADSCs (109.3±10.7 and 97.6±7.6 vs 18.3±1.7, respectively; P<0.01). In addition, conditioned medium from ADSCs promoted the proliferation and invasion of pancreatic cancer cells, and AMD3100, a CXCR4 antagonist, significantly downregulated these growth-promoting effects. We conclude that ADSCs can promote the proliferation and invasion of pancreatic cancer cells, which may involve the SDF-1/CXCR4 axis.
Highlights
It has been increasingly recognized that cancer cells actively recruit stromal cells into tumors and that this recruitment is essential for the generation of a microenvironment that promotes tumor growth [1]
Secretion of stromal cell-derived factor-1 (SDF-1) by adipose tissue-derived stem cells (ADSCs) The amount of SDF-1 secreted by ADSCs and pancreatic cancer (PaCa) cells was determined by ELISA
These data confirmed that ADSCs, but not PaCa cell lines, secrete SDF-1
Summary
It has been increasingly recognized that cancer cells actively recruit stromal cells into tumors and that this recruitment is essential for the generation of a microenvironment that promotes tumor growth [1]. It has been suggested that stromal cells are associated with pancreatic cancer, and play an active role in cancer progression. Pancreatic stroma cells can stimulate tumor growth and invasiveness [2,3], induce chemotherapy resistance, and inhibit apoptosis of cancer cells [4,5]. It has been demonstrated that the SDF-1/ CXCR4 receptor-ligand system plays an important role in pancreatic cancer progression by promoting tumor cell migration and angiogenesis [13,14]
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