Abstract
With the improvement of people's living standards, the number of obese patients has also grown rapidly. It is reported that the level of oxidative stress in obese patients has significantly increased, mainly caused by the increase in reactive oxygen species (ROS) levels in adipose tissue. Studies have shown that the use of siRNA to interfere with bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) expression could promote adipocyte differentiation, and under hypoxic conditions, BAMBI could act as a regulator of HIF1α to regulate the polarity damage of epithelial cells. In view of these results, we speculated that BAMBI may regulate adipogenesis by regulating the level of ROS. In this study, we generated adipose-specific BAMBI knockout mice (BAMBI AKO) and found that compared with control mice, BAMBI AKO mice showed obesity when fed with high-fat diet, accompanied by insulin resistance, glucose intolerance, hypercholesterolemia, and increased inflammation in adipose tissue. Interestingly, adipose-specific deficiency of BAMBI could cause an increase in the expression level of Nox4, thereby promoting ROS production in cytoplasm and mitochondria and the DNA-binding activity of C/EBPβ and ultimately promoting adipogenesis. Consistently, our findings indicated that BAMBI may be a reactive oxygen regulator to affect adipogenesis, thereby controlling obesity and metabolic syndrome.
Highlights
With the improvement of people’s living standards, the number of obese patients has grown rapidly
We tested the effect of bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) on adipogenesis under chow diet (CD), but we found that there were no significant differences in body weight, liver tissue weight, subcutaneous adipose tissue, visceral adipose tissue, adipocyte morphology, and adipogenesis marker genes between the two groups of mice (Fig. S1)
We found that the liver, iWAT, eWAT, and brown adipose tissue (BAT) of the knockout mice were heavier than those of the control mice (Fig. 1, H–L)
Summary
With the improvement of people’s living standards, the number of obese patients has grown rapidly. Adipose-specific deficiency of BAMBI could cause an increase in the expression level of Nox4, thereby promoting ROS production in cytoplasm and mitochondria and the DNA-binding activity of C/EBPβ and promoting adipogenesis.
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