Abstract
The growth plate is cartilage tissue found at the end of long bones in children, responsible for longitudinal bone growth. Injuries to the growth plate cartilage often lead to unwanted bony repair, resulting in growth disturbances such as limb length discrepancy and angulation deformity in children. There is currently no clinical treatment that can fully repair an injured growth plate. Tissue engineering is promising for regeneration of growth plate. Adipose-derived stromal vascular fraction highlight the promising potential as tissue engineering therapy for inducing regeneration of injured growth plate and able to reduce the formation of bony repair that can lead to deformity and limb length discrepancy. Using an animal model of growth plate injury, bone bridge formation is evaluated after 28 days using Enzyme-linked Immunoassay, radiology, histopathology and Immunofloresence examination. Radiological analyses performed by evaluation of grey value using ImageJ software and diameter bone bridge measured from the end to end distance between uninjured growth plate evaluated by histopatology examination. Enzyme-linked Immunoassay and immunofloresence are used to evaluate chondrocyte and chondrogenic marker within the defect. The result shows in group with Adipose-derived stromal vascular fraction have a significant lower bone bridge formation compare to positive control group. This current study represents the first work that has utilized this animal model to investigate whether Adipose-derived stromal vascular fraction can be used to initiate regeneration at the injured growth plate.
Highlights
The growth plate is a cartilaginous tissue located at the proximal and distal ends of the long bones of children, and is responsible for longi tudinal bone growth and regulated process called endochondral ossifi cation [1,2]
The aim of this study is to evaluate the potential of Adipose-derived Stromal vascular fraction as a tissue engineering-based therapies for preventing bony bridge formation on growth plate injury model in rat
The grey values are related to the absorption of x-rays, the radiologic density of a certain tissue which it means the untreated samples have a higher radiological density within the growth plate compare to the negative control group and treated group
Summary
The growth plate is a cartilaginous tissue located at the proximal and distal ends of the long bones of children, and is responsible for longi tudinal bone growth and regulated process called endochondral ossifi cation [1,2]. The growth plate is a common site for trauma injury and being the most fragile area of the growing long bones in children with up to 20% of children’s bone fractures involving growth plate damage [3]. The Salter–Harris classification system has been used to determine and predict the outcome, prognostic factor and severity of a growth plate injury. This classification system divides growth plate injury into five types. 30% of growth plate-related injuries are of types 3, 4 or 5, which affect all of the growth plate layer and the blood supply.
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