Adipose-Derived Stromal Cells Seeded on Integra® Dermal Regeneration Template Improve Post-Burn Wound Reconstruction.

Marcin Piejko,Eliza Zimoląg,Anna Chrapusta,Michał Nessler,Sylwia Bobis-Wozowicz,Karolina Radziun,Zbigniew Madeja,Agnieszka Waligórska,Justyna Drukała

doi:10.3390/bioengineering7030067

Marcin Piejko, Eliza Zimoląg + Show 7 more

Open Access

https://doi.org/10.3390/bioengineering7030067

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Journal: Bioengineering	Publication Date: Jul 2, 2020
Citations: 13	License type: CC BY 4.0

Affiliation: Jagiellonian University

Abstract

Fibrosis of burn-related wounds remains an unresolved clinical issue that leads to patient disability. The aim of this study was to assess the efficacy of the transplantation of adipose-derived stromal cells seeded onto a collagen-based matrix in the reconstruction of burn-related scars. Here, we characterized an in vitro interaction between adipose-derived stromal cells and a collagen-based matrix, Integra®DRT. Our results show that transcription of pro-angiogenic, remodeling, and immunomodulatory factors was more significant in adipose-derived stromal cells than in fibroblasts. Transcription of metalloproteinases 2 and 9 is positively correlated with the collagenolytic activity of the adipose-derived stromal cells seeded onto Integra®DRT. The increase in the enzymatic activity corresponds to the decrease in the elasticity of the whole construct. Finally, we validated the treatment of a post-excision wound using adipose-derived stromal cells and an Integra®DRT construct in a 25-year-old woman suffering from burn-related scars. Scarless healing was observed in the area treated by adipose-derived stromal cells and the Integra®DRT construct but not in the reference area where Integra®DRT was applied without cells. This clinical observation may be explained by in vitro findings: Enhanced transcription of the vascular endothelial growth factor as well as remodeling of the collagen-based matrix decreased mechanical stress. Our experimental treatment demonstrated that the adipose-derived stromal cells seeded onto Integra®DRT exhibit valuable properties that may improve post-excision wound healing and facilitate skin regeneration without scars.

Highlights

Skin and subcutaneous tissue provide accessible sources of regenerative cells such as keratinocytes [1], human skin fibroblasts [2], and adipose-derived stromal cells (ASCs) [3]
Keratinocyte-based therapies have advanced from an experimental to standard treatment of wounds [1], ASCs are considered in numerous medical indications, from the regeneration of perianal fistula and facilitating wound healing to bone reconstruction [2,4,5,6]
For large post-burn scars, collagen-based dermal substitutes such as Integra®DRT [10,11], AlloDerm [12], and Acellular Dermal Matrix [9] are combined with surgical treatment to manage the post-excision area

Summary

Introduction

Skin and subcutaneous tissue provide accessible sources of regenerative cells such as keratinocytes [1], human skin fibroblasts [2], and adipose-derived stromal cells (ASCs) [3]. Keratinocyte-based therapies have advanced from an experimental to standard treatment of wounds [1], ASCs are considered in numerous medical indications, from the regeneration of perianal fistula and facilitating wound healing to bone reconstruction [2,4,5,6]. For large post-burn scars, collagen-based dermal substitutes such as Integra®DRT [10,11], AlloDerm [12], and Acellular Dermal Matrix [9] are combined with surgical treatment to manage the post-excision area

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