Abstract
Objective Adipose-derived stem cells (ADSCs) are attractive source of regenerative medicine, because of their high accessibility with low invasiveness. It's beneficial effects have been reported on liver injury. We investigated the efficacy of ADSCs on the outcome of hepatic ischemia-reperfusion and subsequent hepatectomy in rats. Methods ADSCs were isolated from subcutaneous adipose tissues of rats. After clamping the hepatoduodenal ligament for 15 min, the rats were subjected to 70% partial hepatectomy. After releasing the clamp, 2×106 cells of ADSCs per rat were injected through the penile vein (ADSCs group). As a control, PBS was injected (Sham group). The hepatic regeneration rate was measured on day 1 and 2 after the surgery. The blood and liver samples were obtained for the further analysis. The expression of hepatic regeneration-associated genes in the liver were determined by real-time RT-PCR. Results The hepatic regeneration rates on day 2 were significantly increased in ADSCs compared with Sham. The serum levels of total bilirubin, AST, and ALT did not show any difference between the two groups. Some of the hepatic regeneration-associated genes were significantly upregulated in ADSCs group. Conclusion These results indicated that ADSCs transplantation may provide beneficial effects in the liver following hepatic ischemia-reperfusion and subsequent hepatectomy.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
