Abstract
BackgroundWound healing is impaired in patients with diabetes due to the multifactorial etiology of the disease, which limits the therapeutic efficacy of various approaches. This study hypothesizes that the combination of adipose-derived stem cells (ADSCs) and platelet-rich plasma (PRP) might achieve optimally efficient diabetic wound healing.MethodsADSCs were isolated from the adipose tissues of Sprague-Dawley (SD) rats. PRP was prepared by using a two-step centrifugation technique. A diabetic wound model was established on the backs of SD rats to evaluate the effect of ADSCs incorporated into PRP. Hematoxylin and eosin staining, immunofluorescence, and immunohistochemistry were performed to observe the changes in neovascularization. ELISA and Western blot were utilized to detect the angiogenesis-related protein expression levels. The proliferation of endothelial cells was assessed by the MTS assay.ResultsADSCs incorporated into PRP induced a higher wound closure rate than ADSCs, PRP, and negative control. The expression levels of VEGF, p-STAT3, and SDF-1 in the ADSC+PRP group were higher than those in the other groups. Moreover, the proliferation of endothelial cells was strongly stimulated by treatment with the combination of ADSC-conditioned medium (ADSC-CM) and PRP.ConclusionsPRP enhanced diabetic wound healing induced by ADSCs, and its promoting effect involved neovascularization.
Highlights
Wound healing is impaired in patients with diabetes due to the multifactorial etiology of the disease, which limits the therapeutic efficacy of various approaches
Combined treatment with adipose-derived stem cells (ADSCs) and platelet-rich plasma (PRP) improved diabetic wound healing To determine the optimal concentration of PRP for combination with ADSCs, various concentrations of PRP were placed in the lower chamber of a Transwell plate to attract ADSCs cultured in the upper chamber
We found that 20% PRP induced the greatest migration of ADSCs, and this concentration of PRP was applied in this study (Fig. 2c)
Summary
Wound healing is impaired in patients with diabetes due to the multifactorial etiology of the disease, which limits the therapeutic efficacy of various approaches. This study hypothesizes that the combination of adipose-derived stem cells (ADSCs) and platelet-rich plasma (PRP) might achieve optimally efficient diabetic wound healing. Wound healing is a sequential and overlapping biological process that includes hemostasis, inflammation, growth, reepithelialization, and remodeling. It involves spatial and temporal coordination of multiple cell types and signaling pathways [1]. Some injuries or disease states, such as diabetes, interrupt the normal wound healing progress and result in chronic or nonhealing wounds. Impairment of physiological activities in diabetes delays wound healing and results in chronic wounds. The development of efficient and accessible treatments for chronic wound healing is necessary
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