Adipose derived stem cells and ginkgo biloba extract-loaded PCL/gelatin nanofibrous scaffolds for peripheral nerve injury repair: The impact of physical activity

Abstract

Purpose Peripheral nerve damage can manifest as a result of diverse factors, such as trauma, compression, or underlying diseases. This damage often results in sensory deficits, compromised motor function, and subsequent functional impairments. Methods In the current study, ginkgo biloba extract was loaded into PCL/gelatin scaffolds through electrospinning method. The scaffolds were characterized in vitro using various studies. The prepared nanofibrous scaffolds were rolled up to make neural guidance channels. Then, the conduits were seeded with adipose derived stem cells and transplanted into a rat model of sciatic nerve injury. Results The scaffolds demonstrated non-toxicity and exhibited favorable tensile strength and suture compatibility. Notably, animals subjected to the conduits delivering adipose-derived stem cells in conjunction with ginkgo biloba extract, combined with treadmill exercise, exhibited a marked improvement in both motor and sensory function recovery. Conclusions Furthermore, histopathological examinations have revealed compelling evidence supporting the positive impact of the exercise regimen on the restoration and rehabilitation of the nervous system.