Abstract
BackgroundTo overcome the absorption of traditional fat grafting, techniques for adipose-derived regenerative cell (ADRC)-enriched fat grafting are currently being adapted for practical application. The Celution®800/CRS (Cytori Therapeutics, San Diego, CA) has enabled rapid grafting of the patient’s own freshly harvested ADRCs without requiring a culturing step. However, the optimal cell concentration and the effects of ADRCs on the characteristics of grafted fat after free fat grafting remain unclear.MethodsADRCs were isolated and purified from human fat tissue using the Celution®800/CRS. Animals that received fat grafting without the addition of ADRCs were designated the control group (group A). The number of ADRCs per grafted fat volume (mL) was adjusted to 3 × 105, 1.5 × 106, and 3 × 106 cells/mL (groups B, C, and D, respectively), mixed with free fat, and transplanted as ADRC-enriched fat grafting. These mixtures were transplanted subcutaneously into BALB/C Jcl-nu/nu mice. The volume of grafted fat was determined 5 months after transplantation, and histological assessments were performed.ResultsADRC-enriched fat grafting resulted in decreased fat absorption and the formation of greater numbers of new blood vessels in the grafted fat. The optimal ADRC concentration in this study was found to be 3 × 105 cells/mL (group B), with higher concentrations resulting in increased cyst and fibril formation in the grafted fat.ConclusionsThis study used the Celution®800/CRS for free fat grafting and demonstrated that the concentration of transplanted ADRCs affected the engraftment and quality of the grafted fat.
Highlights
Autologous free fat grafting is a widely accepted technique used for the correction of various soft-tissue defects because it is biocompatible, versatile, natural-looking, nonimmunogenic, inexpensive, and readily obtainable with low donor site morbidity [1,2]
Using the Celution®800/ CRS, 3.0 × 105 ± 5.4 × 104 adipose-derived regenerative cell (ADRC) were extracted from 1 mL of fat
Relative fat volumes were significantly higher in groups B and C than in group A, which showed that fat absorption was inhibited (Figure 5)
Summary
Autologous free fat grafting is a widely accepted technique used for the correction of various soft-tissue defects because it is biocompatible, versatile, natural-looking, nonimmunogenic, inexpensive, and readily obtainable with low donor site morbidity [1,2]. Free fat grafting often has a low survival rate, and adipose tissue can be quickly resorbed and replaced by fibrous tissue and oil cysts [3,4]. To overcome this shortcoming of traditional fat grafting, techniques for adipose-derived regenerative cell (ADRC)-enriched fat grafting are currently. The Celution®800/CRS (Cytori Therapeutics, San Diego, CA) was recently developed to enable rapid grafting of the patient’s own freshly harvested ADRCs by automating and standardizing the extraction, washing, and concentration of ADRCs for clinical use without requiring a culturing step [5]. To overcome the absorption of traditional fat grafting, techniques for adipose-derived regenerative cell (ADRC)-enriched fat grafting are currently being adapted for practical application. The optimal cell concentration and the effects of ADRCs on the characteristics of grafted fat after free fat grafting remain unclear
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
