Adipose derived mesenchymal stem cells efficiently rescue carbon tetrachloride-induced acute liver failure in mouse.

Lisi Deng,Guangze Liu,Bing Liu,Minghua Tong,Xin Wu,Yaping Wang,Kuidong Wang,Yuting Peng,Xiangping Kong

doi:10.1155/2014/103643

Abstract

Background and Aim. Adipose derived mesenchymal stem cells (ADMSCs) may be an attractive source for acute and chronic liver injury because they are abundant and easy to obtain. We aim to investigate the efficacy of ADMSCs transplantation in the acute liver failure (ALF) caused by carbon tetrachloride (CCl4) in mice. Methods. ADMSCs were isolated from inguinal fat pads of enhanced green fluorescent protein (EGFP) transgenic mice and their surface markers and differentiation potential were analyzed. ALF models were established by infusion of CCl4 and divided into two groups: control group; EGFP-ADMSCs transplantation group. The restoration of biological functions of the livers receiving transplantation was assessed via a variety of approaches such as survival rates, live function parameters, histological localization of EGFP-ADMSCs, and Immunofluorescence analysis. Results. ADMSCs were positive for CD105, CD44 but negative for CD45, CD34 and had adipogenic, osteogenic differentiation potential. The survival rate of transplantation group significantly increased compared to PBS group. Furthermore, the transplanted cells were well integrated into injured livers and produced albumin, cytokeratin-18. Conclusion. Direct transplantation of ADMSCs is an effective treatment for ALF. The transplanted ADMSCs exhibit the potential to differentiate into hepatocyte-like cells in the injured livers.

Highlights

Acute liver failure (ALF), with a high mortality rate, is a serious clinical condition
The analysis by flow cytometry revealed that these cells were negative for the expression of hematopoietic markers CD45 and CD34, but positive for CD105 and CD44, which are generally considered as markers of mesenchymal stem cells (MSCs) (Figure 1(b))
The adipogenic differentiation of the Adipose derived mesenchymal stem cells (ADMSCs) was characterized by Oil red O staining, and lipid droplets were visible in the differentiated adipocytes on day 14 after the induction of differentiation (Figure 1(C))

Summary

Introduction

Acute liver failure (ALF), with a high mortality rate, is a serious clinical condition. Adipose derived mesenchymal stem cells (ADMSCs) are an attractive source for regenerative medicine because they are abundant and easy to obtain from liposuction aspirates or excised fat [9]. They display multilineage differentiation potential and can differentiate into hepatocyte-like cells in vitro [10, 11]. We investigated whether the transplantation of ADMSCs is an effective method to prevent ALF in CCl4-induced animal model. Adipose derived mesenchymal stem cells (ADMSCs) may be an attractive source for acute and chronic liver injury because they are abundant and easy to obtain. The transplanted ADMSCs exhibit the potential to differentiate into hepatocyte-like cells in the injured livers

Objectives

Methods

Results

Conclusion