Abstract
Objective: To investigate the role of adiponectin in human glioma cell lines against the temozolomide and the molecular regulation mechanism. Methods: Human glioma cell lines U251 and U-87MG were cultured in Dulbecco’s modified eagle medium (DMEM) containing 4500 mg/L glucose. MTT was used to measure cell growth ratio. Western blot was used to detect the protein levels of autophagy-related protein (Beclin 1, LC3 I/II, p62) and phosphorylated AMPK (p-AMPK) in human glioma cell lines. After AICAR and Compound C were administered, the change of p-AMPK and the autophagy level were examined by western blot. Results: While adiponectin stimulates AMPK in phosphatase and up-regulates the level of autophagy, human glioma cell lines obtain more resistance against the temozolomide, which is facilitated by AICAR and weakened by Compound C. Conclusion: As an important adipokine, adiponectin can up-regulate the glioma cell autophagy by activating the AMPK signaling pathway which increases the resistance of glioma cells to temozolomide.
Highlights
Human Glioma is one of the most common malignancies in central nervous system and accounts for 20% - 40%How to cite this paper: Sun, P., Yan, F., Song, J.N. and Ma, X.D. (2016) Adiponectin Supports Human Glioma Cells Survival against Temozolomide through Enhancement of Autophagic Response in Glioma Cells
While adiponectin stimulates AMPK in phosphatase and up-regulates the level of autophagy, human glioma cell lines obtain more resistance against the temozolomide, which is facilitated by AICAR and weakened by Compound C
As an important adipokine, adiponectin can up-regulate the glioma cell autophagy by activating the AMPK signaling pathway which increases the resistance of glioma cells to temozolomide
Summary
Human Glioma is one of the most common malignancies in central nervous system and accounts for 20% - 40%. (2016) Adiponectin Supports Human Glioma Cells Survival against Temozolomide through Enhancement of Autophagic Response in Glioma Cells. Some studies indicated that, in the hypoxic state, adiponectin could increase the survival rate of rectal cancer cells by up-regulating the level of autophagy [8]. Liu Lei et al [9] proved that autophagy could enhance tumor cells’ resistance to chemotherapeutic drugs. That adiponectin could increase the resistance of glioma cells to temozolomide by up-regulating autophagy needs to be further verified. This study used human glioma cell lines U-87MG and U251 as the research object and detected the role of adiponectin in human glioma cell lines against the temozolomide and the molecular regulation mechanism
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