Abstract
The purpose of this study was to evaluate the role of adiponectin receptor 1 (adipoR1) on bone physiology using porcine AdipoR1 transgenic mice (pAdipoR1) as a model. We found that pAdipoR1 transgenic mice have higher bone mineral density than wild‐type mice in older ages. The percent bone volume and trabecular number determined by micro‐CT, was also significantly higher in pAdipoR1 transgenic mice than in wild‐type female mice at both young and older ages. ELISA analysis revealed that serum osteocalcin (Oc) and osteoprotegrin (OPG) were significantly increased in young pAdipoR1 transgenic mice in both genders. Furthermore, serum OPG also elevated at 8 week and 32 week in female and male pAdipoR1 transgenic mice at young and old ages. Serum TRAP5b concentration was reduced in male pAdipoR1 mice compared with wild‐type mice. Knock‐down AdipoR1 significantly decreased the gene expression of Oc, OPG, alkaline phosphatase and msh homeobox 2 and mineralization in MC3T3‐E1 and mesenchymal stem cells, suggesting that AdipoR1 may mediate the function of bone growth. In addition, we found that AdipoR1 may regulate osteoblasts differentiation through GSK‐3β/β‐catenin signaling by pathscan analysis. We conclude that adipoR1 is a critical factor regulating osteoblast differentiation and bone homeostasis.
Published Version
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