Abstract
Objective and methods: CORS-26 (collagenous repeat containing sequence of 26-kDa protein) was identified as a new gene transcript expressed in cartilage with unknown function. It was the aim to investigate expression, regulation and function of CORS-26 in adipocytes. CORS-26 mRNA and protein expression was investigated by RT-PCR, Western blot analysis and quantitative real-time PCR. Transcriptional regulation was investigated by electrophoretic mobility shift assay (EMSA) and luciferase reporter gene assay (LRA). The adipocytic secretion of adiponectin and resistin was measured by ELISA.
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