Abstract
Background: Mesenchymal stromal cells (MSC) are being tested for the treatment of immune diseases. MSC are present in several adult tissues which milieu may influence MSC behavior particularly under inflammatory conditions. Additionally, culture conditions also can modify cell function or state of activation. Methods: To address the influence of the MSC source on its characteristics, we studied a xenofree, platelet lysate supplemented MSC from dental pulp, adipose tissue and bone marrow, co-cultured with isolated T cells and PBMC subset, and studied the effect of culture animal or human supplements immunomodulatory effect. Results: All three sources were efficient in inhibiting T cells. Among all MSC sources, as also described by others, adipose MSC was capable to significantly induce Treg phenotype and decrease T CD8+. Furthermore, comparing fetal bovine serum and platelet lysate, results demonstrate that platelet lysate alone is capable to induce immunomodulatory phenotype. Additional studies have to be made to elucidate the PL immunomodulatory effect.
Highlights
Mesenchymal stromal cells are a specialized adult stem cell type of mesodermal origin
We explored the immunomodulatory effect of Mesenchymal stromal cells (MSC) from dental pulp of human exfoliated deciduous teeth (SHED), adipose tissue (AD) and bone marrow (BM) with PBMC and magnetically isolated T lymphocytes, as well as the possible effect that FCS and platelet lysate (PL) supplements can affect MSC behavior
There were a total of 10 samples analyzed, three from BM and SHED, and four from adipose tissue
Summary
Mesenchymal stromal cells are a specialized adult stem cell type of mesodermal origin. BM MSC regenerative potential has been explored in pre-clinical and clinical studies focusing on trans-differentiation and tissue engineering [3]. MSC has been identified in most of the adult tissues, like liver; adipose tissue; lungs; heart; dental pulp; muscle; cord blood; placenta, synovial membrane and tendons [6]. MSC are present in several adult tissues which milieu may influence MSC behavior under inflammatory conditions. Methods: To address the influence of the MSC source on its characteristics, we studied a xenofree, platelet lysate supplemented MSC from dental pulp, adipose tissue and bone marrow, co-cultured with isolated T cells and PBMC subset, and studied the effect of culture animal or human supplements immunomodulatory effect. Among all MSC sources, as described by others, adipose MSC was capable to significantly induce Treg phenotype and decrease T CD8+. Additional studies have to be made to elucidate the PL immunomodulatory effect
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