Abstract
The effects of chronic administration of testosterone of α 2-adrenoceptor expression in male hamsters were investigated in order to explore the selectivity of testosterone regulation towards the α 2-adrenoceptor subtypes. A homogeneous population of α 2-adrenoceptors was identified with [ 3H]RX821002 binding in adipocytes, colocytes and liver, whereas the α 2-adrenoceptor sites identified in kidney and brain were heterogeneous. Competition studies with α 2-adrenoceptor ligands characterized the presence of the α 2A-adrenoceptor subtype in adipocytes, colocytes, kidney and brain homogenates and of the α 2B-adrenoceptor subtype in kidney and liver. RNase protection assay with a selective hamster α 2A-adrenoceptor cRNA probe confirmed the expression of α 2A-adrenoceptor mRNA in adipocytes, colocytes, kidney and brain. Testosterone treatment did not modify the α 2-adrenoceptor densities whatever the subtype, except for the adipocyte α 2A-adrenoceptor, which was significantly increased. These results demonstrate that testosterone only up-regulates the adipocyte α 2A-adrenoceptor.
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