Abstract
SUMMARY– The microbial flora of unirradiated and irradiated (0.5 Mrad) Bombay duck (Harpodon nehereus) was differentiated into organisms as spoilers and non‐spoilers based on ability to liquify gelatin; ferment glucose; and produce indole, H2S and urease. In spoiling unirradiated fish, there was a predominance of Vibrio, Aeromonas and Proteus spp while Micrococci and Achromobacter spp were the major surviving groups in irradiated Bombay duck stored for 15 days at 10°C. The predominant spoilers, Proteus vulgaris and Aeromonas hydrophila, produced large amounts of TMA and TVBN and were radiation sensitive as indicated by D10 values of 8.6 and 5.4 Krad respectively. Micrococcus luteus was relatively biochemically inert and radiation resistant, the D10 value being 88 Krad.
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