Adiabatic 1H decoupling scheme for very accurate intensity measurements in 13C NMR

Abstract

Adiabatic proton decoupling has been optimized in order to obtain accurate quantitative measurements of intensities on 13C NMR spectra. For each offset, the minimum adiabaticity factor (Km) reached during the pulse was computed. This K m profile was used to optimize the peak value and the swept frequency range of the adiabatic pulses. With a cosinus amplitude modulation, offset-independent-adiabaticity, and the M4P5–M4P9–M4P5′–M4P9′ phase cycle, an accuracy of 2‰ for the 13C NMR measurements was reached. An approach using bi-labeled 13C acetic acid and ethanol at 99% allowed a fine experimental determination of the uniformity of the decoupling profile. The comparison with WALTZ-16 highlights the improvements in the uniformity of the proton decoupling.