Adhesive interactions between normal and dystrophic human skin fibroblasts

Abstract

The adhesive properties of skin fibroblasts from patients with Duchenne muscular dystrophy (DMD) were studied by analysing cell aggregate formation in suspensions consisting of normal and DMD fibroblasts. By the use of aggregation kinetics and fluorescent labelling, the genotypic composition of aggregates in mixed-cell suspensions could be visualised. The distribution of normal and DMD cells within these aggregates could then be compared to theoretical binomial distributions which assume no difference in the specific adhesiveness between the two genotypes. Analysis of the 3- and 4-cell aggregates which were produced by co-aggregating normal and DMD cells demonstrate that there is no qualitative (specific) difference in the adhesiveness between normal and DMD fibroblasts. However, quantitative changes in the cell-cell adhesion of DMD fibroblasts may be present, and this is supported by the relatively small proportion of intermediate size heterotypic aggregates which were formed in mixed-genotype cell suspensions. In such mixtures, fewer aggregates consisting of 5 or more cells were formed compared to fibroblast suspensions derived from pairs of normal individuals. Furthermore, cell suspensions from pairs of DMD patients produced even less ⩾ 5-cell aggregates than were found in the mixed-genotype experiments. These findings are considered in relation to previous reports of abnormal cell adhesiveness and other adhesion-related mechanisms in DMD cells.