Adhesive characteristics of Mutans Streptococci on the surface of filling materials and sealant

Abstract

Streptococcus mutans (S. mutans) is most commonly founded Mutans Streptococci group in human oral cavity. This study aimed to compare the adhesion ability of Xylitol-sensitive (Xs) and Xylitol-resistant (Xr) on three filling materials which are currently used by dentists. Hydroxyapatite (HA) disk (control group, n=36), sealant (n=36), resin (n=36), glass-ionomer (n=36) disks were made by mold (10.2 mm O, 3 mm hight). Xs and Xr of S. mutans KCTC3065 and S. mutans UA159 were formed and cultured in TYE media. After culture, 500 ㎕ of 0.01% crystal violet was added to the plate, dyeing was performing, and mixed 1 ㎖ solution of ethanol:acetone (4:1 w/w) for reaction termination. The absorbance of the extracted solution was measured by using ELISA reader and the extracted solution on disk was observed with SEM. The adhesion ability was calculated by ELISA reader. Adhesion ability of Xs and Xr from S. mutans of resin was higher than those of HA disk and the adhesion ability of sealant was lower compared to those of HA disk. Sealant had the lowest adhesion ability of dental caries-inducing bacteria among three filling materials when viewed in terms of relevance of the material and decay-inducing bacteria in oral cavity.