Adhesion and Growth of Neuralized Mouse Embryonic Stem Cells on Parylene-C/SiO2 Substrates.

Alan F Murray,Evangelos Delivopoulos

doi:10.3390/ma14123174

Alan F Murray, Evangelos Delivopoulos

Open Access

https://doi.org/10.3390/ma14123174

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Journal: Materials	Publication Date: Jun 9, 2021
Citations: 1	License type: CC BY 4.0

Affiliation: University of Edinburgh, University of Reading

Abstract

Neuronal patterning on microfabricated architectures has developed rapidly over the past few years, together with the emergence of soft biocompatible materials and tissue engineering scaffolds. Previously, we introduced a patterning technique based on serum and the biopolymer parylene-C, achieving highly compliant growth of primary neurons and astrocytes on different geometries. Here, we expanded this technique and illustrated that neuralized cells derived from mouse embryonic stem cells (mESCs) followed stripes of variable widths with conformity equal to or higher than that of primary neurons and astrocytes. Our results indicate the presence of undifferentiated mESCs, which also conformed to the underlying patterns to a high degree. This is an exciting and unexpected outcome, as molecular mechanisms governing cell and ECM protein interactions are different in stem cells and primary cells. Our study enables further investigations into the development and electrophysiology of differentiating patterned neural stem cells.

Highlights

Neuralized Mouse Embryonic StemTissue architecture is one of the most important parameters dictating organ function, where it ranges from simple to very complex.Cell patterning as a research area encompasses multiple techniques to direct cell placement and growth on a variety of 2D and 3D substrates [1]
It is surprising that stem cells respond to adsorbed cell adsorbed cell adhesion proteins on paryelne-C considering that their integrin recepto adhesion proteins on paryelne-C considering that their integrin receptors are strongly deare strongly dependent on their niche and oriented towards facilitating stem c pendent on their niche and oriented towards facilitating stem cell interactions
The main integrin receptor of neural stem cells from the mou the main integrin receptor of neural stem cells from the mouse subventricular zone is α6β1 subventricular zone is α6β1 and responds to laminin [34], whereas the attachment and responds to laminin [34], whereas the attachment of primary neonatal rat astrocytes to primary neonatal rat astrocytes to vitronectin is primarily mediated by integrins αvβ5 a vitronectin is primarily mediated by integrins αvβ5 and α8β1 [35]

Summary

Introduction

Neuralized Mouse Embryonic StemTissue architecture is one of the most important parameters dictating organ function, where it ranges from simple (e.g., kidney) to very complex (e.g., central nervous system).Cell patterning as a research area encompasses multiple techniques to direct cell placement and growth on a variety of 2D and 3D substrates [1]. Cell patterning has enabled biomaterial evaluation [8] and unlocked new modalities in probing specific tissue development and function [9]. SiO2 is hydrophilic and is the most commonly used substrate in microfabrication. It has been used extensively in neuronal patterning [12] and as a dielectric and encapsulant for in vivo neuronal probes [13]. Proteins that adsorb onto parylene-C and SiO2 surfaces have radically different conformational profiles [14]. This is due to the drastically different surface energies of parylene-C and SiO2

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Discussion

Conclusion