Adeponectin gene polymorphism, rs2241766, is associated with coronary artery disease in Iraqi population

Abstract

BackgroundGlobally, coronary artery disease (CAD) is considered to be the first major cause of death and estimated to be 8.1 million in 2013. Angina, myocardial infarction and heart failure represent the three most common forms of CAD. Adiponectin (ADN) is an adipokine predominately secreted from the adipose tissue. It has anti-inflammatory and anti-atherosclerotic properties. Several studies have suggested that adiponectin gene (ADN) single nucleotide polymorphisms (SNPs) is involved in the development of CAD, but with inconsistent results. AimThe aim of the present study is to highlight the association of rs2241766 SNP in exon2 of adiponectin gene with CAD in Iraqi population. MethodsA case control study consisted of 150 patients with CAD attended the cardiology center in Najaf-Iraq and 150 healthy individuals. Selected patients were those of ≥70% narrowing of at least one part of the major coronary artery or its main branches on angiography. Patients and healthy individuals were genotyped for the +45T/G polymorphism using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. ResultsThe frequency of the genotypes of +45T/G SNP was significantly different in patients from those of the control group. The odds ratio of those of the GT genotype was found to be 2.01 (95% CI = 1.10–3.17, P = 0.019) and 6.67 (95% CI = 1.32–33.80, P = 0.022) for those of the GG genotype. The minor allele frequency G was found to be significantly elevated in patients (24.67%) relative to the control group (15.33%).These results suggested a risk factor to develop CAD of 2 and 6.67for carriers of the GT and GG genotypes respectively. ConclusionThe rs2241766 (+45T/G) SNP of adiponectin gene is implicated in the pathogenesis of CAD in Iraqi population with a risk factor of 2 and 6.67 for carriers of the GT and GG genotypes respectively.