Abstract
Four recently described species of new genera of sulfate-reducing bacteria, Desulfobulbus propionicus, Desulfobacter postgatei, Desulfococcus multivorans and Desulfosarcina variabilis were examined with respect to adenylylsulfate reductase. All of the species examined contained the enzyme in sufficient concentrations to account for dissimilatory sulfate reduction. Adenylylsulfate reductase was enriched 17.1-fold from Desulfobulbus propionicus by ammonium sulfate fractionation, ion exchange chromatography and gel filtration. The molecular weight was 175,000 and the enzyme contained 1 mol of flavin, 8 mol of non heme iron and 8 mol of labile sulfide per mol enzyme. Either ferricyanide or cytochrome c could be used as electron acceptors; the pH optimum was 7.7 with ferricyanide and 8.8 with cytochrome c. K m values for AMP and sulfite were 90 μM and 1.3 μM with ferricyanide and 91 μM and 71 μM with cytochrome c as electron acceptor. K m values for ferricyanide and cytochrome c were 89 μM and 21 μM, respectively. The properties of the enzyme were compared with those of purified adenylylsulfate reductases from other microorganisms.
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