Abstract
1. 1. A purification procedure and some of the general properties of adenylate kinase (ATP:AMP phosphotransferase, EC 2.7.4.3) from yeast are given. 2. 2. A fraction of yeast adenylate kinase which was immunologically homogeneous was also homogeneous by the following criteria: column chromatography; paper electrophoresis; moving boundary electrophoresis and ultracentrifugal analysis at two pH's. The molecular weight was determined as 41 000. 3. 3. The pH vs. activity curves and the K m of adenylate kinase from yeast are compared with those of myokinase. 4. 4. The subcellular distribution was determined using two methods of cell disruption. Both methods indicate that the adenylate kinase in yeast is not associated with the particulate fractions of yeast.
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