脳血管障害急性期における血清adenylate kinase活性について(第2報)

Abstract

When the diagnosis of acute cerebral vascular disease is made, CT scan of the brain and other medical instruments, such as NMR or positron CT, have recently become very useful. However, the measurement of enzyme activity in serum, such as creatine phosphokinase and aldolase, is not always useful in such disease. Therefore convenient enzymatic diagnosis of cerebral vascular disease is requested in primary care. We examined CPK isoenzynee of patients who had suffered from acute cerebral infarction and found atypical band at the negative pole side of MM band. As we suspected it was adenylate kinase which is well known as myokinase, we measured it in healthy adults and patients with acute cerebral infarction. The activity of it is the highest in skeletal muscles but it also existed in the brain. The enzyme activity was measured by the decrease of NADH through the production of ADP.The obtained results were as follows: The normal serum adenylate kinase levels were lower than 5mU/ml and the activity in patients with acute cerebral infarction was, on average, 18.79mU/ml. Furthermore the activity of it increased in the early days of cerebral infarction and then decreased gradually. In addition to, we made cerebral infarctions in rabbits experimentally and measured serum AK levels. The activities of AK in serum increased immediately after the treatments in some of them and continued on a high value until the 5th day after onset. But those of the other animals increased gradually after the treatment. Namely it was observed that the measurement of serum adenylate kinase may be useful in the diagnosis of acute cerebral infarction.