Adenylate cyclase-associated protein 1 is associated with metastasis of non-small cell lung cancer especially in brain metastasis patients and promotes the lung cancer cell proliferation and migration in vitro as well as its growth and metastasis in vivo by the signaling pathways of limk1-cofilin

Abstract

Objective: This study was designed to predict non-small cell lung cancer metastasis and explore mechanism of lung cancer cell proliferation, metastasis and migration in vitro and vivo. Methods: Firstly, Adenylate cyclase-associated protein 1, CAP1 were determined by real-time PCR and western blot analysis and IHC in 82 lung cancer specimens. Secondly, BM group (n =50) and non-BM group (n =70) were treated and followed up by 4 years. Thirdly, we characterized the expression of CAP1 in lung cell by using Western blot and RT-PCR analyses. In addition, Si-CAP1, empty vector, over-CAP1 in cultured A549,95-C,95-D by using MTT, wound Healing, Transwell invasive, Western blot, and IHC analyses and nude mouse. Results: We provided evidence that overexpression of CAP1 in lung cancer cells, particularly at BM [figure 1]. In addition,These findings suggest that CAP1 promotes the lung cancer cell proliferation and migration in vitro as well as its growth and metastasis in vivo via LIMK/cofilin I [figure 2]. Conclusion: CAP1 is associated with metastasis of NSCLC especially in BM patients and promotes the lung cancer cell proliferation and migration in vitro and vivo by limk1-cofilin.