Abstract
In order to gain further information concerning the pathogenesis of graft-versus-host disease, the activity of adenylate cyclase (EC 4.6.1.1) was studied in lymphoid and non-lymphoidal organs of animals undergoing graft-versus-host reaction. A marked increase in the adenylate cyclase activity was observed in the washed cell particles isolated from lymph nodes and spleen of animals undergoing regional and systemic graft-versus-host reactions, respectively. Isoproterenol was found to stimulate the enzyme in all the control and experimental preparations; however, NaF failed to stimulate the enzyme in preparations obtained from lymph nodes of rats undergoing regional graft-versus-host reaction. The elevated adenylate cyclase activity in lymph node preparations from rats undergoing graft-versus-host reaction was found to be associated with a decrease in K m value without any significant change in the value for V. The adenylate cyclase activities in the non-lymphoidal organs such as heart, kidney and brain from animals undergoing graft-versus-host reaction were not significantly different from the control values. It is suggested that the observed activation of adenylate cyclase during graft-versus-host reaction may be a direct consequence of cell-cell interaction.
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