Abstract
The basal, serotonin- and fluoride-stimulated activities of homogenate preparations from Hirudo medicinalis segmental ganglia increase with incubation temperature, reaching a maximum at 35°C. Both basal and fluoride-stimulated activities yield linear Arrhenius plots, whereas serotonin-enhanced activity shows a break at 25°C. Increasing Mg 2+ concentrations stimulate the adenylate cyclase both in the absence and in the presence of serotonin. The affinity of the enzymatic system for the cation is unaffected by the amine.
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