Abstract
Methods Receptor usage was assessed using CD46 transgenic mouse cells, as well as by CARand CD46-specific mAb blocking studies using human PBMC. Twelve adult rhesus monkeys were inoculated with of 10 viral particles (vp) of replication-competent Ad5 and Ad26 (N=6) or saline (N=6) at weeks -8 and -4, and were vaccinated intramuscularly with 3×10 vp replication-incompetent Ad26-Gag/Pol/Env vectors. At week 2, monkeys were sacrificed to assess immunologic and inflammatory responses at mucosal surfaces.
Highlights
Adenovirus serotype 5 (Ad5) utilizes coxsackievirus and adenovirus receptor (CAR) as its primary cellular receptor
Transduction by Ad26 and Ad35 vectors was markedly enhanced in CD46 transgenic mouse cells compared with wild type mouse cells
Transduction of human PBMC by Ad26 and Ad35 vectors was efficiently blocked by the anti-CD46 mAbs 13/42, M177 and MEM-258, but not by the anti-CAR mAbs RmcB and E1-1
Summary
Adenovirus serotype 5 (Ad5) utilizes coxsackievirus and adenovirus receptor (CAR) as its primary cellular receptor. Adenovirus serotype 26 utilizes CD46 as primary cellular receptor and only transiently activates T lymphocytes following vaccination of rhesus monkeys H Li1*, EG Rhee1, K Masek-Hammerman2, JE Teigler1, P Abbink1, DH Barouch1
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