Adenovirus-mediated Ink4a/ARF gene transfer significantly suppressed the growth of pancreatic carcinoma cells

Abstract

Objectives: To determine the effects of adenovirus-mediated transfer of p14ARF and p16INK4a on growth and apoptosis of human pancreatic carcinoma cell lines. Results: Pancreatic carcinoma cell lines, PC-7, PANC-1 and MIA PaCa-2 (p14ARF and p16INK4a -/-), were used. PC-7 (p53 wt) and MIA PaCa-2 (p53 mt) cells infected with recombinant adenovirus vector expressing p14ARF gene (Adp14) showed significant inhibition of cell growth compared with control vector in vitro and in vivo, whereas PANC-1 cells (p53 mt) did not show such effects. Those three cell lines exhibited growth retardation and senescence phenotype after infected with the adenovirus vector containing p16INK4a gene (Adp16) in vivo and in vitro. Conclusions: Adenovirus-mediated transfer of p14ARF and p16INK4a produces significant growth suppression of pancreatic carcinoma cells in vitro and in vivo. The effects of Adp14 partly depend on the status of p53 gene in those cells.