Abstract
The binding characteristics of [ 3H]nitrobenzylthioinosine ([ 3H]NBI) to rat brain membrane preparations was examined, and the autoradiographic distribution of this ligand in brain sections was compared with the immunohistochemical localization of adenosine deaminase (ADA). It was found that [ 3H]NBI labels sites for which adenosine has far higher affinity than do other nucleosides, that these sites are heterogeneously distributed and that there is an exact correspondence between areas containing [ 3H]NBI sites and ADA-immunoreactive neurons. Our results indicate that [ 3H]NBI and ADA are potential markers for revealing anatomical sites at which actions of adenosine may be expressed.
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