Abstract
DURING investigations on the specific adrenal medullary granules containing adrenaline and nor-adrenaline1, it was found that these structures have high contents of acid-labile phosphorus. In preliminary experiments with ion-exchange chromatography on ‘Dowex 2’ using formic and hydrochloric acid as eluting agents, a fraction was obtained in which the main part of the acid-labile phosphorus was recovered. Judging from the ultra-violet absorption and the ratio pentose: acid-labile phosphorus : total phosphorus, the chief compound in this fraction seemed to be adenosine triphosphate. Therefore the method of Cohn and Carter2 for separation of adenosine phosphates was applied to adrenal medullary extracts.
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