Adenosine triphosphate bioluminescence assay for monitoring contamination of the working environment of anaesthetists and cleanliness of the operating room.

Abstract

Anaesthetists possibly contribute to the spread of infections during anaesthesia. The adenosine triphosphate (ATP) bioluminescence assay is an easy-to-perform, on-the-spot assay that provides objective data; therefore, using the LuciPac®Pen and the Lumitester PD-20®System, we assessed contamination of the working environment of anaesthetists before and after surgery as well as their hands at the time of each procedure during induction and extubation. Similarly, cleanliness of the operating room was evaluated using this assay to determine whether it is useful to assess the effectiveness of the routine cleaning protocols followed after surgery. ATP concentrations in the working environment of anaesthetists and their hands increased during surgery. In addition, ATP concentrations within the working environment decreased after routine cleaning with ethanol or accelerated hydrogen peroxide; however, there were no differences in the number of sites with ATP concentrations >500 relative light units before and after cleaning. This method is useful to evaluate contamination of the working environment of anaesthetists; nevertheless, it is prudent to evaluate the effectiveness of routine cleaning protocols because ATP bioluminescence assays are influenced by the use of various disinfectants at varying concentrations.