Adenosine deaminase production by an endophytic bacterium (Lysinibacillus sp.) from Avicennia marina.

Kandasamy Kathiresan,Muthu Sivasankaran,Sunil Kumar Sahu,Kandasamy Saravanakumar

doi:10.1007/s13205-013-0144-2

Abstract

The present study was carried out with the following objectives: (1) to isolate the endophytic bacilli strains from the leaves of mangrove plant Avicennia marina, (2) to screen the potential strains for the production of adenosine deaminase, (3) to statistically optimize the factors that influence the enzyme activity in the potent strain, and (4) to identify the potent strain using 16S rRNA sequence and construct its phylogenetic tree. The bacterial strains isolated from the fresh leaves of a mangrove A. marina were assessed for adenosine deaminase activity by plating method. Optimization of reaction process was carried out using response surface methodology of central composite design. The potent strain was identified based on 16S rRNA sequencing and phylogeny. Of five endophytic strains, EMLK1 showed a significant deaminase activity over other four strains. The conditions for maximum activity of the isolated adenosine deaminase are described. The potent strain EMLK1 was identified as Lysinibacillus sp. (JQ710723) being the first report as a mangrove endophyte. Mangrove-derived endophytic bacillus strain Lysinibacillus sp. EMLK1 is proved to be a promising source for the production of adenosine deaminase and this enzyme deserves further studies for purification and its application in disease diagnosis.

Highlights

Endophytic bacteria are important source for developing the novel drugs for effective treatment of diseases in humans, plants and animals (Strobel et al 2004)
The present study was carried out with the following objectives: (1) to isolate the endophytic bacilli strains from the leaves of mangrove plant Avicennia marina, (2) to screen the potential strains for the production of adenosine deaminase, (3) to statistically optimize the factors that influence the enzyme activity in the potent strain, and (4) to identify the potent strain using 16S rRNA sequence and construct its phylogenetic tree
The potent strain EMLK1 was identified as Lysinibacillus sp. (JQ710723) being the first report as a mangrove endophyte

Summary

Introduction

Endophytic bacteria are important source for developing the novel drugs for effective treatment of diseases in humans, plants and animals (Strobel et al 2004). Adenosine deaminaseADA (EC 3.5.4.4) is a zinc-metallo enzyme involved in purine metabolism (Alrokayan 2002, 2007). This enzyme is widely distributed in different species (Bachrach 2004; Pospisilova 2007) but not reported from mangrove endophytes. The genes encoding these deaminases are essential in bacteria and yeast (Losey et al 2006). This enzyme in particular is of special interest for its role in cellular growth regulation and differentiation (Hershfield and Mitchell 1995). The present study was attempted to isolate endophytic bacilli from the mangrove plant A. marina for exploring the production of adenosine deaminase and to identify the potent strain by using 16S rRNA sequencing

Methods

Results

Conclusion