Abstract
Activation of A 2A adenosine receptors (A 2A-Rs) was found to prevent reactive oxygen species (ROS) formation and apoptosis in serum-deprived pheochromocytoma PC12 cells. A protein kinase A (PKA) inhibitor not only blocked the anti-apoptotic roles of A 2A-R but also reversed A 2A-R-induced suppression of ROS formation, indicating a PKA-dependent pathway in preventing ROS formation and apoptosis. PKA stimulators and antioxidants prevented serum-deprived ROS formation and apoptosis. In addition, A 2A-R activation also prevented H 2O 2-induced cell death, further suggesting the protective roles of A 2A-R in antagonizing oxidative stress and cell death. Finally, antioxidative system-interrupting agents attenuated A 2A-R-mediated protection. Taken together, these data indicate that PKA-dependent regulation and attenuation of oxidative stress by A 2A-R may play at least some roles in preventing serum-deprived PC12 cell apoptosis.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
