Abstract
During early development, adenosine contributes to the occurrence of respiratory depression and recurrent apneas. Recent physiological studies indicate that GABAergic mechanisms may be involved in this inhibitory action of adenosine, via their A 2A receptors. In the present study, in situ hybridization with ribonucleotide probes for A 2A receptor (A 2AR) mRNA was combined with the immunolabeling technique for parvalbumin and transneuronal retrograde tracing method using green fluorescent protein expressing pseudorabies virus (GFP-PRV) to (1) characterize age-dependent changes in the expression of adenosine A 2ARs mRNA in brain stem regions where GABAergic neurons are located; (2) determine whether GABA-containing neurons express A 2AR mRNA traits, and (3) identify whether bulbospinal GABAergic neurons projecting to phrenic nuclei contain A 2AR mRNA. Results revealed expression of A 2A receptors in regions of medulla oblongata containing GABAergic neurons, namely in the ventral aspect of the medulla, within the Bötzinger region and caudal to it, the gigantocellular reticular nucleus, midline neurons and the caudal ventrolateral medulla oblongata. Furthermore, a subpopulation of identified GABAergic neurons, projecting to the phrenic motor nuclei, possess A 2AR mRNA. It is concluded that adenosine A 2ARs expressed by GABAergic neurons are likely to play a role in mediating adenosine-induced respiratory depression.
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