Adenosine 3′,5′-cyclic phosphate phosphodiesterase of corpus luteum

Abstract

1. 1. Adenosine 3′,5′-cyclic phosphate diesterase (Ado-3′,5′-P phosphodiesterase) has been demonstrated in human, rat and bovine corpora lutea, and some properties of the bovine enzyme have been studied. 2. 2. Bovine luteal Ado-3′,5′-P phosphodiesterase is present in the 100 000 × g supernatant of tissue homogenate. 3. 3. The mean K m of the enzyme is 0.25 mM and it is subject to inhibition by methylxanthines. 4. 4. Corpora lutea which are at the stage of regression contain more Ado-3′,5′-P phosphodiesterase than those which are collected earlier in the estrous cycle. 5. 5. A preliminary partial purification of the enzyme is described, and its properties are discussed in relation to the properties of Ado-3′,5′-P phosphodiesterases from other mammalian tissues.