α-ADDUCIN GENE AND ESSENTIAL HYPERTENSION IN CHINA

Abstract

Adducin is a membrane skeletal protein that is involved in the regulation of membrane ion transport and cellular signal transduction. Essential hypertension has been linked to α-adducin gene locus, and association of a polymorphism of the gene has been found in some studies, but results of linkage or association studies on α-adducin gene are controversial among different populations. This study was designed to examine the linkage between α-adducin gene locus and essential hypertension and to reveal the relationship between an α-adducin gene polymorphism (Gly460Trp) and essential hypertension in a Chinese population. For the linkage study, one hundred and six Chinese nuclear families were recruited, including 417 hypertensive patients in all 474 individuals. Those samples were genotyped at D4S412 and D4S3038. The distances between the two microsatellite markers and the α-adducin gene locus are less than 3cM. Parametric, non-parametric linkage (NPL) analyses using the GENEHUNTER software were carried out. Sib transmission-disequilibrium test (S-TDT), as well as transmission-disequilibrium test (TDT), was also implemented with TDT/S-TDT Program 1.1. Serum levels of uric acid, creatinine, blood urea nitrogen (BUN), fasting glucose and lipids were determined as phenotypes. In an association study, 138 hypertensive and 121 normotensive subjects were genotyped at Gly460Trp of the α-adducin gene to examine a possible association between this polymorphism and blood pressure or other phenotypes. We fail to find the linkage between the two markers and essential hypertension by parametric, NPL analysis or TDT/S-TDT study. With the use of the simple association and the multivariate logistic regression analyses, we also fail to reveal a significant association between the Gly460Trp polymorphism in α-adducin gene and the blood pressure variation, or blood biochemical indices studied. The frequency of the 460Trp allele in Chinese (46–48%) is similar to that found in Japanese (54–60%) while the allele frequency is less common in Caucasian (13%–23%). These findings suggest that in our Chinese population, α-adducin 460Trp variant may not play an important role in the etiology of EH. And the negative results of linkage and TDT/S-TDT further supports this conclusion.