Additive Positive Effects of Pollination on Bt and Non-Bt Soybean Cultivars by Honey Bee and Native Flower-Visiting

Fabiola De Oliveira,Marcos Gino Fernandes

doi:10.5539/jas.v13n1p157

Abstract

In addition to the increase in the number of pods produced, a well-conducted pollination also contributes to an increase in the number of grains per pod, improves the quality of the grains and seeds, and renders the grain ripening more uniform, thus, increasing the production at harvest. The objective of the present study was to quantify the benefits of flower-visiting insects in soybean production. The experiment was conducted with two soybean cultivars, one Bt and one non-Bt. During the flowering period, 150 plants of each cultivar were randomly selected, yielding 25 replicates (three plants per replicate) with free access to flower visitors, and another 25 replicates (also with three plants per replicate) that flower visitors could not access. During the flowering period, 549 specimens of flower-visiting insects were found in both cultivars, divided into eight orders, 30 families, and 92 species. The most abundant species were Apis mellifera (Linnaeus) (Hymenoptera: Apidae), Musca sp.1 (Linnaeus) (Diptera: Muscidae), and Lagria villosa (Fabricius) (Coleoptera: Tenebrionidae). In the treatment with flower visitors, grain weight increased by 84.22% in Bt cultivar and by 202.52% in non-Bt cultivar, compared with the area without the presence of flower visitors. The increase in the number of pods in Bt and non-Bt cultivars was 45.72% and 101.25% respectively, in the area open to flower visitors. The high increase in grain yield and number of pods observed in the area with free access to pollinator insects emphasizes the high importance of the pollination service performed by flower visitors to the soybean crop.

Highlights

Soybean (Glycine max (Linnaeus) Merrill) is a very important crop worldwide; it is one of the main agricultural commodities in Brazil—its second largest producer in the world—where the area cultivated with this legume has increased continuously (Agrianual, 2009; Embrapa, 2015)
Despite the clear benefits of the use of pesticides in agriculture—avoiding losses associated with insect attack—there is a persistent need to develop additional alternatives or technologies that allow the rational use of these products, and provide adequate protection for a sustainable production of food, feed, and fiber (Sharma et al, 2000)
Genetic engineering has resulted in the creation of genetically modified (GM) varieties of many cultivars that express the Bacillus thuringiensis (Berliner, 1915) (Bacillales: Bacillaceae) (Bt) toxin

Summary

Introduction

Soybean (Glycine max (Linnaeus) Merrill) is a very important crop worldwide; it is one of the main agricultural commodities in Brazil—its second largest producer in the world—where the area cultivated with this legume has increased continuously (Agrianual, 2009; Embrapa, 2015). Heavy application of pesticides to control crop pests can adversely affect beneficial organisms, leave chemical residues in food, and cause environmental pollution. Despite the clear benefits of the use of pesticides in agriculture—avoiding losses associated with insect attack—there is a persistent need to develop additional alternatives or technologies that allow the rational use of these products, and provide adequate protection for a sustainable production of food, feed, and fiber (Sharma et al, 2000). The ingestion of the Cry protein by the insects triggers an osmotic rupture of their digestive tract epithelial cells, culminating in their death before they are able to damage the crop (Hof & Whitely, 1989; Sankula, 2006; Andow, 2008)

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Conclusion