Additive effects of two quantitative trait loci that confer Rhopalosiphum maidis (corn leaf aphid) resistance in maize inbred line Mo17.

M Betsiashvili,K R Ahern,G Jander

doi:10.1093/jxb/eru379

M Betsiashvili, K R Ahern + Show 1 more

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https://doi.org/10.1093/jxb/eru379

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Abstract

Plants show considerable within-species variation in their resistance to insect herbivores. In the case of Zea mays (cultivated maize), Rhopalosiphum maidis (corn leaf aphids) produce approximately twenty times more progeny on inbred line B73 than on inbred line Mo17. Genetic mapping of this difference in maize aphid resistance identified quantitative trait loci (QTL) on chromosomes 4 and 6, with the Mo17 allele reducing aphid reproduction in each case. The chromosome 4 QTL mapping interval includes several genes involved in the biosynthesis of DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one), a maize defensive metabolite that also is required for callose accumulation in response to aphid feeding. Consistent with the known association of callose with plant defence against aphids, R. maidis reproduction on B73×Mo17 recombinant inbred lines was negatively correlated with both DIMBOA content and callose formation. Further genetic mapping, as well as experiments with near-isogenic lines, confirmed that the Mo17 allele causes increased DIMBOA accumulation relative to the B73 allele. The chromosome 6 aphid resistance QTL functions independently of DIMBOA accumulation and has an effect that is additive to that of the chromosome 4 QTL. Thus, at least two separate defence mechanisms account for the higher level of R. maidis resistance in Mo17 compared with B73.

Highlights

Cultivated maize (Zea mays) is a genetically diverse crop plant that exhibits wide variation in its resistance to insect herbivores (McMullen et al, 2009a; Meihls et al, 2012)
The chromosome 4 quantitative trait loci (QTL) mapping interval includes several genes involved in the biosynthesis of DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one), a maize defensive metabolite that is required for callose accumulation in response to aphid feeding
Discovery of the actual genetic basis of such insect resistance QTL has been facilitated by the genome sequence of maize inbred line B73 (Schnable et al, 2009), as well as by populations of recombinant inbred lines (RILs) and nearisogenic lines (NILs) that have been created from B73 and a diverse set of other maize inbred lines (Eichten et al, 2011; Lee et al, 2002; McMullen et al, 2009b; Yu et al, 2008)

Summary

Introduction

Cultivated maize (Zea mays) is a genetically diverse crop plant that exhibits wide variation in its resistance to insect herbivores (McMullen et al, 2009a; Meihls et al, 2012). Genetic mapping of this difference in maize aphid resistance identified quantitative trait loci (QTL) on chromosomes 4 and 6, with the Mo17 allele reducing aphid reproduction in each case.

Results

Conclusion